Fremgangsmåte til å fremstille heterologe proteiner i Pichia pastoris ved bruk av ekspresjonsvektorer inneholdende sekvenser som induseres av metanol

摘要

Novel DNA sequences which are responsive to the presence of methanol, catabolite non-repressing carbon sources and carbon source starvation are provided. In addition, novel constructs including these DNA sequences, as well as transformed organisms therewith are provided. Processes for producing the DNA sequences and constructs of the invention are detailed. The production of polypeptide product under the control of the regulatory regions of the invention is demonstrated.