Enhanced fluorescence detection of samples in capillary column

摘要

A simple device for collecting a greater amount of fluorescent emission from a minute sample in a capillary column. An axially symmetrical paraboloid reflector is implemented to collect fluorescent emission from the capillary column. The reflector also serves as a simple bracket for positioning and aligning the capillary column. Fluorescent emission is collimated by the paraboloid reflector which allows more effective use of band pass filters in blocking scattered radiation from detection. The scattered and transmitted radiation can be used to facilitate alignment of the capillary with respect to the detection optics. The paraboloid reflector also facilitates the implementation of simultaneous multiple-channel detection.