| 摘要 |
PURPOSE:To provide a printing method using an enzyme, hardly being restricted by kinds of dyes used, almost free from damage to a substrate, hardly causing ooze out of colored boundary part, exhibiting shaded color or bright color and capable of readily coloring and decoloring and to obtain an enzyme for decoloring an azo-based dye enabling the printing. CONSTITUTION:A liquid or a sizing agent (which may contain a non-azo-based dye) containing at least an azoreductase (an enzyme belonging to diaphorase) produced by a bacterial cell, Bacillus OY1-2 (FERM P-13118) of a bacterium belonging to the genus Bacillus among azo-based dyes, non-azo-based dyes and this enzyme is applied in desired form onto a dyed substrate dyed with an azo-based dye (which may contain non-azo-based dye) and the coloring is lost or reduced by reacting the enzyme with the dye, and then, printing (direct printing, white discharge printing, colored discharge printing or resist printing) is carried out. A substrate which is not dyed can also be combined with a solution, etc., containing the enzyme and the azo-dye, etc. |
