Immunogenic compositions prepared from adenylate cyclase of B. Parapertussis

摘要

The invention concerns an immunogenic composition characterised in that it contains an adenylate cyclase (AC) preparation, obtainable from <Underline>B. parapertussis</Underline>, said adenylate cyclase preparation comprising an active adenylate cyclase having a molecular weight (<Underline>Mr</Underline>) of about 43 ± 4.3 - 45 ± 4.5 kDa as determined with respect to ovalbumin as standard, specifically recognized by specific polyclonal and monoclonal <Underline>anti-B. pertussis</Underline> AC antibodies, and capable of antigenically cross-reacting with the <Underline>B. pertussis</Underline> AC, and/or an inactive protein having a molecular weight (<Underline>Mr</Underline>) of about 43 ± 4.3 kDa, specifically recognized by polyclonal anti-AC antibodies raised against purified AC preparation and devoid of adenylate cyclase calmodulin-activable activity and of affinity for calmodulin.