Expression system, integration vector and cell transformed by this integration vector

摘要

Expression system (A) for extracellular prodn. of a microbial aspartic protease (I) comprises: (i) a promoter; (ii) a secretion signal sequence; (iii) propeptide sequence; (iv) sequence encoding mature (I) and (v) a terminator. Also new are: (1) integration vector contg. (A); (2) filamentous fungus cells transformed by this vector; (3) the isolated and purified strain Aspergillus foetidus SE4tra; (4) the promoter and terminator of A. foetidus SE4 glucoamylase (their sequences of 2054 and 1035 bp, respectively, are reproduced in the specification) and (5) (I) produced by the transformed cells.