NOVEL BACILLUS THURINGIENSIS STRAINS ACTIVE AGAINST LEPIDOPTERAN AND COLEOPTERAN PESTS

摘要

The invention is related to a novel delta-endotoxins, each having a molecular weight of about 33,000. The delta-endotoxins are obtainable from a novel biologically pure Bacillus thuringiensis (B.t.) strains active against lepidopteran and coleopteran pests which produces a bipyramidal crystal consisting essentially of at least two delta-endotoxins having a molecular weight of about 130,000 daltons and a rhomboidal crystal consisting essentially of two delta-endotoxins, each having a molecular weight of about 33,000 daltons and/or mutants thereof. The invention also relates to insecticidal compositions obtainable therefrom. The invention further relates to methods of using the insecticidal compositions to control an insect pest(s) from the order Lepidoptera and/or Coleoptera. The invention also relates to isolated nucleic acid sequences encoding the delta-endotoxins and probes for detecting such nucleic acid sequences.