| 摘要 |
<p>The present invention is drawn to novel toxin genes purified and isolated from Bacillus thuringiensis var kurstaki and given the designations CrylE(c) and CrylE(d). The novel toxin genes encode proteins of approximately 130 kDa in size and are active against Lepidopteran insects. Also included in the invention are the proteins encoded by CrylE(c) and CrylE(d). Further disclosed are recombinant genes and proteins derived from CrylE(c) and CrylE(d). Also provided are biologically pure bacterial strains transformed with at least one of the novel toxin genes which can be used in entomocidal formulations for the control of Lepidopteran insects. Yet another aspect of the invention are plants transformed with at least one of the toxin genes or active fragments thereof, particularly where the transforming sequences have been optimized for expression in maize.</p> |
