摘要 |
A method for detecting Helicobacter pylori is disclosed which method involves contacting a sample suspected of containing Helibacter pylori with a medium which provides for substantially selective growth of Helicobacter pylori, incubating the sample with the medium for a time sufficient for detection of Helicobacter pylori growth and detecting the growth and thereby reducing the presence of Helicobacter pylori within the sample. The methodology employs a wide range of a different culture mediums which are modified specifically for the selective growth and specific detection of Helicobacter pylori. A typical medium includes Columbia broth supplemented with urea and a pH indicator. The methodology provides for a relatively high degree of sensitivity (i.e., small numbers of bacteria present within a sample are detected) as well as high selectivity (i.e., the method provides for a low percentage of false positives). Various kits used in connection with the method are designed so that they can be used by unskilled users in an "at home" setting. The kits and methodology are economical, easily used and provide highly accurate results within a relatively short period of time (e.g., 3 days or less).
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