TRANSFORMATION OF GUAR

摘要

The invention relates to a method for the transformation and regeneration of legumes of the genus Cyamopsis, in particular Agrobacterium-mediated transformation of guar (Cyamopsis tetragonoloba, by introducing a recombinant DNA sequence into at least one cell or protoplast and generating genetically modified explants using at least one selection or shoot growth medium comprising at least one compound selected from an auxin inhibitor, e.g. 2-(p-chlorophenoxy)-2-methylpropionic acid (PCIB), a beta -lactamase inhibitor, e.g. sulbactam, and an ethylene inhibitor, e.g. silver thiosulfate, so as to obtain a genetically modified plant or part thereof containing in its genome at least one recombinant DNA sequence; to genetically modified plants produced by the method; and to the use of substances such as the beta -lactamase inhibitor sulbactam to facilitate transformation of guar and other plants.