发明名称 |
NUCLEIC ACID SEQUENCES AND EXPRESSION SYSTEMS FOR HEPARINASE II AND HEPARINASE III DERIVED FROM FLAVOBACTERIUM HEPARINUM |
摘要 |
The present invention describes the isolation and sequence of genes from Flavobacterium heparinum encoding heparin and heparan sulfate degrading enzymes, heparinase II and heparinase III (EC 4.2.2.8). It further describes a method of expressing and an expression for heparinases I, II and III using a modified ribosome binding region derived from a promoter from glycosaminoglycan lyase genes of F. heparinum. Also, a multi-step protein purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography and hydroxylapatite chromatography is outlined. Antibodies against a post-translational modification moiety common to Flavobacterium heparinum proteins and a method to obtain antibodies specific to these moieties and to the amino acid sequences of heparinases I, II and III are described.
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申请公布号 |
WO9534635(A1) |
申请公布日期 |
1995.12.21 |
申请号 |
WO1995US07391 |
申请日期 |
1995.06.09 |
申请人 |
IBEX TECHNOLOGIES;ZIMMERMANN, JOSEPH |
发明人 |
ZIMMERMANN, JOSEPH;SU, HONGSHENG;BLAIN, FRANCOISE;BENNETT, CLARK;GU, KANGFU;MUSIL, ROY |
分类号 |
C12N15/09;C07K16/40;C12N1/21;C12N9/88;C12N15/60;C12N15/74;C12P21/08;C12R1/19;(IPC1-7):C12N1/21;C12N15/63;C12N15/67;C12N15/70 |
主分类号 |
C12N15/09 |
代理机构 |
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地址 |
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