| 摘要 |
Microsatellite nucleotide repeat sequences present in DNA isolated from a biological sample are used to determine the origin of a biological sample by a process including the steps of isolating nucleic acid from a biological sample, determining the interspersion pattern of repeats of a nucleotide sequence selected from the group consisting of di-, tri-, and tetra-microsatellite nucleotide repeat sequences in the DNA, and comparing the determined interspersion pattern with known interspersion patterns of the nucleotide sequence in selected mammalian species. Species-specific, individually-invariant interspersion patterns of di-, tri-, or tetra-microsatellite nucleotide repeats in vertebrate DNA samples are identified by a process including the step of annealing separated strands with an oligonucleotide primer selected from the group consisting of di-, tri- and tetra-microsatellite nucleotide repeat sequences at a concentration ratio of primer to DNA template of less than about 2500:1, the annealing being conducted at a temperature greater than about 30 DEG C and in the presence of magnesium ions at a concentration less than about 3.0 mM, to produce an extension product of the primer which is complementary to the strand of the DNA sample.
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