Two Hepatitis C Virus envelope proteins (E1 and E2) are expressed without sialylation. Recombinant expression of these proteins in lower eukaryotes, or in mammalian cells in which terminal glycosylation is blocked, results in recombinant proteins which are more similar to native HCV glycoproteins. When isolated by GNA lectin affinity, the E1 and E2 proteins aggregate into virus-like particles.
申请公布号
WO9533053(A1)
申请公布日期
1995.12.07
申请号
WO1995US06072
申请日期
1995.05.15
申请人
CHIRON CORPORATION
发明人
RALSTON, ROBERT, O.;MARCUS, FRANK;THUDIUM, KENT, B.;HALL, JOHH, A.;BERGER, KIM, M.;CHOO, QUI-LIM;HOUGHTON, MICHAEL;KUO, GEORGE;GERVASE, BARBARA, A.
