SIMPLE METHOD AND DEVICE FOR IMMUNOCHEMICAL ASSAY

摘要

The invention provides an assay method comprising an immunochemical qualitative or quantitative (semi-quantitative) analysis according to chromatography conducted by dissolving a marker serving as an index for judgement in a sample containing the object of analysis, allowing the sample and the marker to migrate to the site (detecting section) for reading the result of judgement on the chromatographic material, and visually judging the result of analysis, more specifically a simple immunochemical assay method (invention I) characterized by laminating a marker-containing member with a member (a marker elution accelerating member) made from a raw material that is equally coarse with or coarser than the marker-containing member. The invention also provides a simple semi-quantitative immunochemical assay method using a hapten as the object of analysis according to chromatography (invention II) including the indispensable constituent features of invention I and characterized by bringing (1) the object of analysis in a sample and (2) the marker-containing object of analysis or a chemical modification thereof into contact with (3) an antibody against the object of analysis, which antibody is immobilized on one and the same chromatographic material in various concentrations independently from the upstream side to the downstream side to thereby effect a competitive antigen-antibody reaction sequentially from the upstream side to the downstream side, and determining the concentration of the object of analysis in a sample by using the marker as an index. The invention further provides an assay device having such a construction as to accomplish the inventions I and II. According to the above method and device, it is possible in an immunochromatographic assay (qualitative or semi-quantitative analysis) to obtain clear and accurate assay results in a short time by allowing the sample and the marker to migrate on the chromatographic material synchronously. Further the invention provides an immunochemical method and a stick-shaped semi-quantitative device capable of conducting semi-quantitative analysis of a hapten-containing sample readily in a short time and clearly.