发明名称 |
Separation-free specific binding assays using anti-inhibitor antibodies. |
摘要 |
<p>Specific binding ligands can be detected with an assay which utilizes an immobilized receptor for the ligand, a reporter enzyme, an inhibitor antibody and an anti-inhibitor antibody. Both antibodies are specific for the reporter enzyme. The inhibitor antibody effectively shuts down the activity of the reporter enzyme when it is complexed thereto. The anti-inhibitor antibody binds to the reporter enzyme, does not affect the enzymatic activity, but prevents the binding of the inhibitor enzyme. This assay provides a direct correlation of the target specific binding ligand to the signal generated without the use of separation or wash steps.</p> |
申请公布号 |
EP0685740(A2) |
申请公布日期 |
1995.12.06 |
申请号 |
EP19950303655 |
申请日期 |
1995.05.30 |
申请人 |
JOHNSON & JOHNSON CLINICAL DIAGNOSTICS, INC. |
发明人 |
DAISS, JOHN L.;GORMAN, KEVIN M.;HINCHMAN, CAROLYN R. |
分类号 |
G01N33/531;C12Q1/00;C12Q1/28;G01N33/543;G01N33/58;(IPC1-7):G01N33/543;G01N33/94 |
主分类号 |
G01N33/531 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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