发明名称 |
Process for the purification of homogeneous platelet-derived endothelial cell growth factor from a platelet lysate. |
摘要 |
<p>A process for the purification of homogeneous plate-derived endothelial cell growth factor (PD-ECGF) from a platelet lysate comprises subjecting the lysate to cation exchange chromatography on CM-Sephadex, pooling and subjecting the resulting active fractions to anion exchange chromatography on QAE-Sephadex, pooling and subjecting the resulting active fractions to ammonium sulfate precipitation, pooling and subjecting the resulting active fractions to anion exchange chromatography on DEAE-Sepharose, pooling and subjecting the resulting active fractions to high-performance hydroxylapatite column chromatography, pooling and subjecting the resulting active fractions to high performance hydrophobic column chromatography on an alkyl-Superose resin, and recovering said homogeneous platelet-derived endothelial cell growth factor. The process, which can be carried out in five steps, allows for purification of PD-ECGF that results in about 1,250,000-fold purification at a yield of about 14%.</p> |
申请公布号 |
EP0685490(A1) |
申请公布日期 |
1995.12.06 |
申请号 |
EP19950108643 |
申请日期 |
1989.12.15 |
申请人 |
LUDWIG INSTITUTE FOR CANCER RESEARCH |
发明人 |
HELDIN, CARL-HENRIK;MIYAZONO, KOHEI;WERNSTEDT, CHRISTER;HELLMAN, ULF;TAKAKU, FUMIMARO;ISHIKAWA, FUYUKI |
分类号 |
C12N1/21;A61K38/00;A61P7/00;A61P9/10;A61P43/00;C07K1/20;C07K14/475;C07K16/00;C07K16/22;C12N15/16;C12P21/02;C12P21/08;C12R1/19;(IPC1-7):C07K14/475;C07K1/16 |
主分类号 |
C12N1/21 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|