QUALITATIVE SURFACE IMMUNOASSAY USING CONSECUTIVE REAGENT APPLICATION

摘要

Diagnostic devices, and methods for their use in the rapid detection of antibody analytes are provided. The subject devices comprise an assay pathway having a wicking strip (10), a sample pad (12), a detection region (14) comprising at least one detection (16) and control (20) zone, and an absorption strip (22). The detection zone (16) provides an epitope of an antigen of interest for binding to the antibody analyte, while the control zone (20) provides for binding to non-specific antibodies in the sample. A conjugate of a receptor and colloidal particle is employed for detection, whose arrival at the detection zone (16) is subsequent to that of the sample, so as to allow for binding of the antibody in the detector (16) and control (20) zones and removal of non-specifically bound antibody from the detection zone (16). The presence of a signal in the detection zone (16) indicates a positive result.