摘要 |
Assay in a sample of a bloodgroup antigen present on erythrocytes or an antibody binding to such a bloodgroup antigen. The sample is treated with a reagent containing antibody binding to a bloodgroup antigen, or erythrocytes, whereby a complex of erythrocyte, bloodgroup antigen present thereon and antibody bound thereto is formed if the reagent contains antibody binding to a bloodgroup antigen present in the sample or the sample contains antibody binding to a bloodgroup antigen present in the reagent. This complex, if any has formed, is separated from uncomplexed erythrocytes by filtration through a solid phase which is permeable to the uncomplexed erythrocytes, on which phase an immunoglobulin-binding material is immobilized, whereafter the result is detected. The filtration can be effected by means of centrifugation. The method can be used for bloodgroup assay, antibody screening and identification, and cross tests. |