METHOD AND APPARATUS USEFUL FOR DETECTING BLOODGROUP ANTIGENS AND ANTIBODIES

摘要

The direct assay comprises adding a sample of erythrocytes to a reaction tube charged with a column of immunoreactive particles having an immunoglobulin binding ligand selected from the group consisting of Protein A, Protein G, Protein A/G or a universal kappa light chain binding protein coupled to the surface of the particles. Antibodies specific for bloodgroup antigens tested for are coupled to the ligand on the particles. The indirect assay comprises obtaining either a sample of erythrocytes or a sample of blood serum to be tested and mixing the erythrocytes or serum with a known antibody or antigen reagent, depending on whether antigens or antibodies are being tested for. The mixture is incubated in a reaction tube above a column of immunoreactive particles having immunoglobulin binding ligands selected from the group consisting of Protein A, Protein G, Protein A/G or a universal kappa light chain binding protein coupled to the surface of the particles. A positive response is indicated by binding of the erythrocytes to the immunoreactive particles in the upper one-half of the reaction tube. A negative response is indicated by the erythrocytes being forced to the bottom of the reaction tube in a pellet.