| 摘要 |
The present invention concerns a method for the identification from DNA of a fragment comprising a simple tandem repeat locus comprising the steps of: i) contacting a DNA library with at least one hybridisation probe so as to identify a population of DNA fragments enriched for simple tandem repeats; ii) isolating and cloning said population; and iii) screening of the resulting DNA library so as to identify an individual fragment comprising a simple tandem repeat locus. Also provided are simple tandem repeats isolated by the method of the present invention, characterised in that they may be amplified at least in part by PCR using a specified pair of primers, together with amplification primers and probes specific to the simple tandem repeats so isolated. The present invention also provides methods of genetic characterisation using the aforementioned simple tandem repeats, primers and probes. |
