发明名称 |
RECOMBINANT PROTEINS OF FILAMENTOUS HAEMAGGLUTININ OF BORDETELLA, PARTICULARLY BORDETELLA PERTUSSIS, METHOD FOR PRODUCING SAME, AND USESTHEREOF FOR PRODUCING FOREIGN PROTEINS OR VACCINATING ACTIVE PRINCIPLES |
摘要 |
A recombinant DNA containing a sequence (1) coding for a polypeptide heterologous to a filamentous haemagglutinin of Bordetella (Fha) fused within the same reading frame to a sequence (2) located upstream from the first sequence. Said sequence (2) codes for at least a part of the Fha precursor, which part comprises at least the N-terminal region of a truncated mature Fha protein which contains the interaction site of Fha and heparin, and which, when it is itself subjected on its own to the control of a promoter recognised by the cell polymerases of B. Pertussis, and inserted into a B. Pertussis cell culture, is expressed in said culture under the control of said promoter and excreted into the cell culture medium. The invention uses both the abilities of Bordetella, and particularly B. Pertussis, which does not appear to produce extracellular proteases, and the ease with which filamentous haemagglutinins can be isolated from those of the Bordetella synthesising same.
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申请公布号 |
CA2202639(A1) |
申请公布日期 |
1995.10.26 |
申请号 |
CA19952202639 |
申请日期 |
1995.04.19 |
申请人 |
INSTITUT PASTEUR DE LILLE |
发明人 |
MENOZZI, FRANCO;CAPRON, ANDRE;LOCHT, CAMILLE;JACOB-DUBUISSON, FRANCOISE;RENAULD, GENEVIEVE;RIVEAU, GILLES |
分类号 |
C07K14/235;C12N15/31;(IPC1-7):C12N15/62;A61K39/00;C07K19/00;A61K39/10;C12N1/21;C07K1/22;C12N1/36;C12N15/74 |
主分类号 |
C07K14/235 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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