METHOD OF ELIMINATING GENETIC ROUTES FOR BACTERIOPHAGE EVOLUTION AND PRODUCTS PRODUCED THEREBY

摘要

-45- The present invention provides a method of identifying a bacterial DNA sequence capable of recombining with DNA of a predetermined bacteriophage the method comprises the steps of: (a) introducing a bacteriophage defense mechanism into a bacterium; (b) culturing the bacterium in the presence of a first lytic bacteriophage to produce a bacterial culture, the first lytic bacteriophage being sensitive to the bacteriophage defense mechanism; (c) isolating from the bacterial culture a second lytic bacteriophage which is resistant to the bacteriophage defense mechanism; (d) identifying DNA sequences present in the second lytic bacteriophage which are not present in the first lytic bacteriophage; and then (e) identifying a bacterial DNA sequence which is homologous to a DNA sequence of the second lytic bacteriophage identified in step (d); wherein the identified bacterial DNA sequence is capable of recombination with DNA of the first lytic bacteriophage.