| 摘要 |
PURPOSE:To obtain thermostable beta-agarase capable of readily producing agar oligosaccharide industrially by cultivating a bacterium belonging to the genus Pseudomonas, having specific physical and chemical properties, capable of producing beta-agarase. CONSTITUTION:A bacterium belonging to the genus Pseudomonas, having the following physical and chemical properties, capable of producing beta-agarase is cultivated and the beta-agarase is collected from the culture mixture. (a) Action: hydrolyzing beta-1, 4 bond of agarose, rapidly reducing viscosity of agarose substance and forming mainly neoagarotetraose and neoagarohexaose. (b) Substrate specificity: acting on galactan polysaccharide such as agarose having beta-1,4 galctoside bond and on oligosaccahrides of >=neoagarooctaose, slightly acting on neoagarobexaose and not acting on lactose. Having other given physical and chemical properties of (c) optimum pH and pH stability, (d) optimum temperature and thermostability, (e) condition of deactivation, (f) molecular weight, (g) isoelectric point and (h) influence of metallic salt. |
