A method of purifying a hydrophobic polypeptide by high-performance liquid chromatography using a mobile phase comprising a solvent mixture wherein the content of trifluoroacetic acid is 3-10% (v/v) and a packing material comprising polyvinyl alcohol. This method is useful because a lung surfactant prepared from the hydrophobic polypeptide purified by this method has a surface activity superior to that of the hydrophobic polypeptide purified by the conventional methods.
申请公布号
EP0652227(A4)
申请公布日期
1995.10.25
申请号
EP19940914582
申请日期
1994.04.28
申请人
TOKYO TANABE COMPANY LIMITED
发明人
TAKEI, TSUNETOMO, TOKYO TANABE COMPANY LIMITED;AIBA, TOSHIMITSU, TOKYO TANABE COMPANY LIMITED;SAKAI, KAORU, TOKYO TANABE COMPANY LIMITED;FUJIWARA, TETSURO