发明名称 |
Method and composition for increasing the accumulation of squalene and specific sterols in yeast |
摘要 |
A method of increasing the accumulation of squalene and specific sterols in yeast comprising increasing the expression level of a structural gene encoding a polypeptide having HMG-CoA reductase activity in a mutant yeast having single or double defects in the expression of sterol biosynthetic enzymes is provided. The expression level of a structural gene is preferably increased by transforming yeast with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity and a promoter that is suitable for driving the expression of the encoded polypeptide in the transformed yeast. The polypeptide having HMG-CoA reductase activity is preferably a truncated, active HMG-CoA reductase enzyme. Recombinant DNA molecules useful for transforming yeast and mutant yeast transformed with such recombinant DNA molecules are also disclosed.
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申请公布号 |
US5460949(A) |
申请公布日期 |
1995.10.24 |
申请号 |
US19910783861 |
申请日期 |
1991.10.28 |
申请人 |
AMOCO CORPORATION |
发明人 |
SAUNDERS, COURT A.;WOLF, FRED R.;MUKHARJI, INDRANI |
分类号 |
C12N1/19;C12N9/04;C12N15/09;C12N15/53;C12N15/81;C12P5/02;C12P33/00;C12R1/865;(IPC1-7):C12N9/02;C12P33/16 |
主分类号 |
C12N1/19 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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