The present invention relates to a method of forming a three-stranded DNA molecule wherein each strand of the three-stranded DNA molecule is hybridized (that is, non-covalently bound) to at least one other strand of the three-stranded DNA molecule. The method comprises: contacting a recombination protein with a double-stranded DNA molecule and with a single-stranded DNA molecule sufficiently complementary to one strand of the double-stranded DNA molecule to hybridize therewith, which contacting is effected under conditions such that the single-stranded DNA molecule hybridizes to the double-stranded molecule so that the three stranded DNA molecule is formed.
申请公布号
US5460941(A)
申请公布日期
1995.10.24
申请号
US19930089910
申请日期
1993.07.12
申请人
THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES
发明人
CAMERINI-OTERO, RAFAEL D.;MCINTOSH, MARGARET;CAMERINI-OTERO, CAROL S.;FERRIN, LANCE J.