PURIFICATION OF OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATES USING ANION EXCHANGE CHROMATOGRAPHY

摘要

The present invention provides improved methods for separating and purifying oligonucleotide phosphorothioates. For the first time, we used DMAE ion-exchange chromatography to separate and purify oligonucleotide phosphorothioates. We found that by varying the conditions employed excellent separation can be obtained. In particular, we have been able to use a higher pH and lower salt concentrations than previously reported in the literature to obtain chromatographic separation of oligonucleotide phosphorothionates having length up to about 35 nucleotides.