发明名称 |
Ein Verfahren zur Herstellung eines Antikörpers, der zur Bestimmung der Anwesenheit eines menschlichen Oncogens befähigt ist. |
摘要 |
Experiments designed to define the differences between the 21 oncogene of DNA isolated from human bladder cancer cells and its corresponding proto-oncogene. By a series of in vitro recombinations, the difference was initially isolated to a 350 kb segment of DNA; sequencing defined the difference as a change in the Gly<1><2> codon causing the p21 protein of the oncogene to contain valine at a location where the p21 protein of the proto-oncogene contained glycine. Assays for detecting carcinogenesis based on such differences are also described, in one type of assay, a restriction enzyme specific for either the altered or non-altered DNA segment of the genes are employed to detect carcinogenesis. In another type of assay, serological reagents, such as antibody specific for either p21 protein expressed from the proto-oncogene or oncogene, or a common site therein, are described. |
申请公布号 |
DE3382767(T2) |
申请公布日期 |
1995.10.19 |
申请号 |
DE19833382767T |
申请日期 |
1983.09.29 |
申请人 |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY, CAMBRIDGE, MASS., US |
发明人 |
WEINBERG, ROBERT A., BROOKLINE MASSACHUSETTS 02146, US;TABIN, CLIFFORD JAMES, CAMBRIDGE MASSACHUSETTS 02139, US;BRADLEY, SCOTT M., MCLEANOEVIRGINIA 22101, US |
分类号 |
A61K39/395;A61K39/00;C07H21/04;C07K14/435;C07K16/00;C07K16/30;C07K16/32;C07K19/00;C12N15/00;C12N15/09;C12P21/00;C12P21/08;C12Q1/68;G01N33/50;G01N33/53;G01N33/531;G01N33/574;G01N33/577;(IPC1-7):C07K16/00;C12N15/02 |
主分类号 |
A61K39/395 |
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