| 摘要 |
PURPOSE:To obtain a new creatine amidinohydrolase, useful as reagents for determining creatine and creatinine, excellent in thermal stability, having a low Km value for the creatine and good in reactivity. CONSTITUTION:This creatine amidinohydrolase is obtained by culturing Alcaligenes faecalis TE3581 (FERM P-14237), etc., and has the following properties: (1) reacting with creatine and producing sarcosine and urea; (2) optimum temperature: about 40-45 deg.C; (3) optimum pH: about 8.0-9.0; (4) stable at <= about 50 deg.C when kept warm at pH7.5 for 30min; (5) stable at pH about 5-8 when preserved at 40 deg.C for 18hr; (6) about 15.2mM value of Km for creatine; (7) molecular weight: about 67000 (measured by the gel filtration method) and about 43000 [measured by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)] and (8) isoelectric point: about 3.5. |
