.ALPHA.-1-ANTICHYMOTRYPSIN ANALOGUES HAVING CHYMASE INHIBITING ACTIVITY

摘要

The invention provides analogues of .alpha.-1-antichymotrypsin having amino acid substitutions at positon 358. .alpha.-1-antichymotrypsin analogues having amino acid substitutions at positions 356-361 and analogues having amino acid substitutions at positions 356-361 wherein the amino acid at position 358 is substituted are also within the scope of the invention. These analogues exhibit chymase inhibitory activity. Also provided are novel .alpha.-1-antichymotrypsins having an N-terminal extension of methionine-alanine-serine or alanine-serine. Expression vectors for the production of .alpha.-1-antichymotrypsins are also provided. The present invention also provides host cells and cell cultures capable of expressing analogues of .alpha.-1-antichymotrypsins, as well as protein preparations from the host cells. Methods of producing and using the analogues of .alpha.-1-antichymotrypsins to inhibit chymase activity are also provided.