| 摘要 |
The present invention relates to constructing expression plasmid pSTE-SK-1 by using streptokinase vectors, such as pLY-4, and transforming E. coli with such plasmid, then inducing the expression of the r-SK gene by heating. The engineered bacteria are propagated by fermenting and destructed, collected inclusion body by centrifugalizing the r-SK product is purified via a two-step method after renaturation. The production and purity of the invention are high and cost low.
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