HIGHLY-PURIFIED RECOMBINANT REVERSE TRANSCRIPTASE

摘要

A plasmid for expression of Moloney Murine Leukemia Virus-dirived reverse transcriptase in E. Coli cells deficient in the expression of indigenous RNAse activity, a method for purification of the recombinant enzyme, and a composition comprising a cloned and purified reverse transcriptase optimized for use in cDNA and nucleic acid amplification procedures.