| 摘要 |
Methods for culturing and isolating fetal cells, e.g., fetal erythroid cells, from a sample of peripheral blood obtained from a pregnant woman are disclosed. Cells from the sample of peripheral blood are cultured in a medium containing a cell growth factor, e.g., an erythroid growth factor, e.g., erythropoietin, to culture fetal cells. Prior to culture, non-nucleated cells can be removed from the sample of peripheral blood and/or fetal cells can be enriched. Metaphase spreads can be prepared from cultured fetal cells. A fetal cell marker or a nucleic acid sequence of interest can be detected in cultured fetal cells. The cultured fetal cells can be used to determine fetal sex and to detect disease-causing mutations and chromosomal abnormalities in a fetus.
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