Compounds and reagents for determination of reticulocytes.

摘要

Quaternized derivatives of acridine orange are used for quantitatively determining reticulocyte levels in whole blood by fluorescence flow cytometry techniques. The quaternized derivatives of acridine orange are of the general formula: wherein X is a benzyl group of formula wherein R1 is hydrogen or fluorine, and R2 is fluorine, trifluoromethyl or hydrogen; or X may be a hydroxyethylene group; and gamma <-> is bromide or iodide.