| 摘要 |
PURPOSE:To obtain a new substance with the core protein as the main constituent inert to human hepatitis virus antibody while having high antigen specificity only to the proteins derived from alien genes, thus useful as a human hepatitis virus antibody test reagent, etc. CONSTITUTION:This fused protein is obtained by incorporating host cell with a recombinant gene produced by linking a human hepatitis virus gene into the core antigen gene of duck hepatitis B virus(DHBV). Specifically, this fused protein can be obtained by the following processes: the DHBV antigen gene obtained from DHBV positive serum is amplified and then treated with a restriction enzyme, and the resultant gene is inserted into a plasmid vector to obtain a manifestation vector having multicloning site; subsequently, a human hepatitis B virus(human HBV) antigen gene treated with a restriction enzyme after amplification is inserted into said multicloning site to obtain a manifestation vector for manifesting the human HBV antigen gene, and finally, this vector is transfected into a host cell such as Escherichia coli followed by making the host cells produce the aimed protein. |
