NOVEL ALKALINE CELLULASES

摘要

Cellulases selected from the group consisting of Family 7 cellulases and variants of these cellulases comprising a core and optionally a C-terminal link consisting of 10 amino acids at the most, especially cellulases having tryptophan, tyrosine or phenylalanine in position 55 relative to the sequence alignment of Figure 5 and/or cellulases having a substrate binding cleft of a depth of at least 12 ANGSTROM , exhibit enhanced enzyme activity in the alkaline pH range while exerting a moderate cellulolytic action on the cellulosic substrate and are, for example, useful in detergent compositions, especially for soil removal or colour clarification or preventing backstaining; in fabric softeners; for bio-polishing of textiles; for drainage improvement of paper pulp; for plant degradation. Cellulases from Humicola insolens, Fusarium oxysporum, Trichoderma reesei and Myceliophthora thermophile are referred to.