发明名称 |
MODIFYING NUCLEOTIDE ANALOGUES |
摘要 |
Fluorescently labelled dideoxynucleoside triphosphates are widely used as chain-terminators in Sanger dideoxy sequencing operations. But these unincorporated dye-terminators migrate in an electrophoresis gel and obscure the desired sequence ladder. This invention provides a method and a kit for modifying the unincorporated dye-terminators, e.g. by removal of a 5'-triphosphate group by chemical or enzymatic means e.g. by use of a phosphatase enzyme.
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申请公布号 |
WO9524505(A1) |
申请公布日期 |
1995.09.14 |
申请号 |
WO1995GB00490 |
申请日期 |
1995.03.07 |
申请人 |
AMERSHAM INTERNATIONAL PLC |
发明人 |
REEVE, MICHAEL, ALAN;ROBINSON, PHILIP, STEVEN |
分类号 |
C07H21/00;C12Q1/42;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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