YEAST STRAINS AND MODIFIED ALBUMINS

摘要

Albumin, for example human albu- min, is expressed and secreted in yeast which has been mutated to lack the yeast aspartyl protease 3 (Yap3p) or its equiva- lent, thereby reducing the production of a 45kD albumin fragment. A further reduc- tion is achieved by additionally deleting the Kex2p function. Alternatively, a modified albumin is prepared which is not suscep- tible to Yap3p cleavage, for example hu- man albumin which is R410A, K413Q and K414Q.