Quantitative determination of pyruvic acid and quantitative analysis for component of living body making use of such determination

摘要

Disclosed herein is a quantitative determination of pyruvic acid, which comprises reacting oxidized nicotinamide adenine dinucleotide, a pyruvate dehydrogenase complex and coenzyme A to a specimen and measuring the amount of the resulting reduced nicotinamide adenine dinucleotide. A quantitative analysis for a component of a living body, in which the quantitative determination is used, is also disclosed. Pyruvic acid or other components existing in a living body or formed in the course of a reaction in the living body can be quantitatively determined simply and precisely.