UBIQUITIN CARRIER ENZYME E2-F1

摘要

Purified isolated E2-F1 enzyme (I) does not bind to anion exchange resin in neutral pH and is capable of stimulating protein degradation of non N-end rule substrates and N-alpha -acetylated substrates. Also claimed are: (1) an antibody which is capable of binding specifically to E2-F1 enzyme; (2) a hybridoma cell line which produces the antibody of (1); (3) a purified and isolated DNA molecule coding for E2-F1 enzyme where the DNA is pref. in the form of an expression vector; (4) a host transformed with the expression vector of (3); and (5) the prodn. of (I) by (a) culturing a recombinant host comprising a gene coding for (I); and (b) isolating (I) from the recombinant host.