| 摘要 |
PURPOSE: To obtain the subject composition containing a specific homogeneous mature recombinant human interleukin-2 and a carrier material, excellent in the potentiating action for fluid and cellular immune reaction, and useful for treating malignant neoplastic diseases and immunodeficiency diseases, etc. CONSTITUTION: This pharmaceutical composition contains (A) a homogeneous mature recombinant interleukin-2 (IL-2) and (B) a carrier material; wherein, the component A has a specific activity of 53×10<6> U/mg determined by the standard CTLL cell<3> H-Tdr uptake assay and is obtained by the following process: microorganisms transformed by a DNA sequence coding for mature human IL-2, mature human IL-2 is expressed and accumulated in the resultant cultured microorganism, the resulting transformed microorganism is lysed to yield a cell lysate mixture, from which the cell membrane component is separated, the IL-2 is extracted from the cell membrane component with a 4-7M guanidine-HCl-contg. was solution, and the IL-2 is purified by chromatography from the resultant was solution. |
