| 摘要 |
<p>A method and apparatus for incorporating macromolecules into membrane vesicles, cells or tissue by electroporation is presented. The method involves the steps of: a) applying a train of low unipolar or alternating voltage pulses to the macromolecules and cells, b) increasing the concentration of the macromolecules at the surface of the cells, and c) allowing the macromolecules to penetrate into the cytosol of the cells through the destabilized cell membrane. The apparatus includes a support plate (36) having apertures for allowing electrodes (22) to pass therethrough. Support tubes (34) located within the apertures surround the electrodes (22). An electric current supplying mechanism (32) connected to the electrodes (22) supplies sufficient electrical current to a cell for facilitating the introduction of macromolecules into the cell.</p> |
