| 摘要 |
Isolated 2-5A-dependent RNases, an inteferon-induced enzyme which is activated by 5'-phosphorylated, 2',5'-linked oligoadenylates (2-5A) and implicated in both the molecular mechanisms of interferon action and in the fundamental control of RNA stability in mammalian cells, and encoding sequences therefor are disclosed. The expression cloning and analysis of murine and human 2-5A-dependent RNases is also disclosed. In addidon, recombinant nucleotide sequences, recombinant vectors, recombinant cells and antiviral plants which express, for example, 2-5A-dependent RNase, 2-5A synthetase and/or double-stranded RNA dependent protein kinase (PKR), or other amino acid sequences which have activity that interferes with or inhibits viral replication are disclosed.
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