发明名称 |
Methods of identifying inhibitors of cdc25 phosphatase |
摘要 |
The present invention makes available assays and reagents for identifying anti-proliferative agents, such as mitotic and meiotic inhibitors, especially inhibitors of cdc25 phosphatase. The present assay provides a simple and rapid screening test which relies on scoring for positive cellular proliferation as indicative of anti-mitotic or anti-meiotic activity, and comprises contacting a candidate agent with a cell which has an impaired cell-cycle checkpoint and measuring the level of proliferation in the presence and absence of the agent. The checkpoint impairment is such that it either causes premature progression of the cell through at least a portion of a cell-cycle or inhibition of normal progression of the cell through at least a portion of a cell-cycle, but can be off-set by the action of an agent which inhibits at least one regulatory protein of the cell-cycle in a manner which counter-balances the effect of the impairment.
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申请公布号 |
US5443962(A) |
申请公布日期 |
1995.08.22 |
申请号 |
US19930073383 |
申请日期 |
1993.06.04 |
申请人 |
MITOTIX, INC. |
发明人 |
DRAETTA, GIULIO;COTTAREL, GUILLAUME;DAMAGNEZ, VERONIQUE |
分类号 |
G01N33/15;A61K38/45;A61K45/00;A61P43/00;C12N1/15;C12N1/19;C12N9/16;C12N15/09;C12Q1/18;C12R1/645;G01N33/50;(IPC1-7):C12Q1/02;C12Q1/42;C12Q1/68 |
主分类号 |
G01N33/15 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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