| 摘要 |
The present specification describes a method of determining effectiveness of a sterilization process which includes the steps of i) contacting a sample to be sterilized and a biological indicator comprising microbial spores with a sterilant to give exposed spores, ii) contacting the exposed spores with a medium selected to germinate the spores, iii) determining a rate of germination of the exposed spores to determine the effectiveness of the sterilization process. The germination rate of the exposed spores may be readily determined using spectrophotometric techniques with or without staining of germinated spores. The present method is applicable for different types of known sterilants including but not limited to steam, ethylene oxide, radiation, heat, sodium hypochlorite, polyvinylpyrrolidone-iodine, sodium dichlorocyanurate, low temperature steam-formaldehyde, glutaraldehyde and hydrogen peroxide, hydrogen peroxide plasma or mixtures thereof. The specification also describes a biological indicator system which uses the present method.
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