FLUORESCENT SPECTRAL DIFFERENTIAL MEASUREMENT OF A SUBSTRATE COATING

摘要

A fluorescent spectrophotometer (100) measures the fluorescent characteristics of fluorescent probes within functional compositions of a specimen. The fluorescent spectrophotometer (100) emits light appropriate for exciting the fluorescer or fluorescers contained within the specimen. The excited fluorescer emits within an emission wavelength spectrum. Fluorescers having a narrower wavelength region over which an abrupt change in emission intensity occurs are chosen for use as a fluorescent probe. The fluorescent spectrophotometer (100) detects the change in intensity through this narrower area and performs a derivative calculation on the intensity curve. The fluorescent spectrophotometer (100) is able to distinguish the fluorescer from other fluorescers or interfering fluorescent background.