发明名称 |
RECOMBINANT DNA MOLECULE CAPABLE OF PRODUCING TUMOR NECROSIS FACTOR IN INCREASED YIELD |
摘要 |
A process of producing mammalian tumor necrosis factors (TNF) and TNF-like polypeptides by culturing prokaryotic hosts transformed with a recombinant DNA molecule comprising DNA sequences encoding those polypeptides operatively linked to either one of the T4 or the pL-T4 expression control sequences. A process for purifying TNF-like polypeptides using an anion exchanger. The TNFs and TNF-like polypeptides produced by the processes of this invention, and compositions and methods utilizing those TNFs and TNF-like polypeptides, are useful in anticancer, antitumor and antimalarial therapies. They are also useful together with interferon therapy and chemotherapy in anticancer and antitumor therapies, and in combination with actinomycin D in the treatment of tumor-bearing mammals. |
申请公布号 |
JPH07213292(A) |
申请公布日期 |
1995.08.15 |
申请号 |
JP19950026272 |
申请日期 |
1995.01.19 |
申请人 |
BIOGEN INC |
发明人 |
FUAIAAZU UORUTAA SHII;FURANSEN RUSHIA EMU;TABERUNIA JIYAN HONOORU REO;MARUMENAUTO ANII RUISU MARII;BAN DERU HEIDEN JIYOZE;ARETSUTO BERUNAARU;KAWASHIMA ERITSUKU EICHI |
分类号 |
G01N33/577;A61K;A61K38/00;A61K38/19;A61K38/21;A61K45/00;A61P33/02;A61P35/00;C07H;C07H21/00;C07H21/04;C07K;C07K1/18;C07K14/00;C07K14/52;C07K14/525;C07K14/555;C07K14/56;C07K14/565;C07K14/57;C12N;C12N15/00;C12N15/09;C12N15/28;C12N15/70;C12P;C12P21/00;C12P21/02;C12Q1/02;C12R1/19 |
主分类号 |
G01N33/577 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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