BIOLUMINESCENT INDICATOR BASED UPON THE EXPRESSION OF A GENE FOR A MODIFIED GREEN-FLUORESCENT PROTEIN

摘要

A pre-coelenterazine peptide comprising a modified A. victoria GFP having an amino acid sequence in which Ser<65> is replaced with Tyr. There are further provided a polynucleotide encoding the pre-coelenterazine peptide, allowing synthesis of large, pure amounts of coelenterazine, as by culturing organisms transformed with the polynucleotide; methods for synthesizing coelenterazine; and improved assays employing the polynucleotide or transformed organisms, e.g., to detect mutagenesis.