发明名称 |
PRODUCTION OF PHARMACEUTICAL-GRADE PLASMID DNA |
摘要 |
The invention relates to a method for producing plasmid DNA comprising the steps of: (a) lysing cells containing the plasmid DNA to obtain a lysate; (b) treating the lysate by a means for removing insoluble material to obtain a solute; and (c) applying the solute to differential PEG precipitations and chromatography to purify the plasmid DNA. In other embodiments of the invention, the plasmid DNA is produced with GRAS reagents; the plasmid DNA is produced in the absence of enzymes; the plasmid DNA is produced in the absence of organic extractants; the plasmid DNA is produced in the absence of mutagens; the lysing, treating and applying steps are scalable to result in the large scale manufacture of the plasmid DNA; and the lysing, treating and applying steps result in the generation of pharmaceutical grade material.
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申请公布号 |
CA2179603(A1) |
申请公布日期 |
1995.08.10 |
申请号 |
CA19952179603 |
申请日期 |
1995.01.09 |
申请人 |
VICAL INCORPORATED |
发明人 |
MARQUET, MAGDA;HORN, NANCY;MEEK, JENNIFER;BUDAHAZI, GREGG |
分类号 |
C12N15/09;C07K14/74;C12N1/12;C12N1/13;C12N15/10;C12P21/02;C12R1/19;C12R1/91;(IPC1-7):C12N15/64;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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