Enzyme participating in c-terminal amidation, and method of preparing same and use thereof.

摘要

An enzyme catalyzing the conversion of a peptide C-terminal glycine adduct represented by the following formula (I): (wherein A represents a residue other than alpha -amino group or imino group and alpha -carboxylic group derived from naturally occurring alpha -amino acid, X represents hydrogen atom or a residue of an amino acid derivative which is bonded to N atom through carbonyl group) to a peptide C-terminal alpha -hydroxyglycine adduct represented by the following formula (II): (wherein A and X have the same meanings as above), and further catalyzing the conversion of the peptide C-terminal alpha -hydroxylglycine adduct represented by the above formula (II) to a C-terminal amidated compound represented by the following formula (III): (wherein A and X have the see meanings as defined above), which comprises culturing host cells transformed with an expression plasmid comprising a DNA coding for said enzyme, and recovering both or either of said enzymes from the culture, characterized in that in said DNA a portion corresponding to the membrane spanning region has been excised.