| 摘要 |
<p>A method of determining the base sequence of single-stranded nucleic acid, including: a first step of hybridizing each of plural primers comprising oligonucleotide, with a template comprising a single-stranded nucleic acid to be examined, thereby to form a complex having a double-stranded portion comprising a portion of the template and the primer, the plural primers being separately disposed in a plurality of regions in accordance with the kinds of the primers; a second step of substantially simultaneously adding plural kinds of nucleotides or nucleotide analogues to the complexes respectively disposed in the plural regions, thereby to perform growth reaction of the primer with the nucleic acid to be examined as the template in a direction of from 5' to 3' of the primer, the plural kinds of nucleotides or nucleotide analogues being complementary to nucleotides constituting the template in the complex, and being capable of forming base pair with nucleotides constituting the template; and a third step of detecting the amount of the growth of the primers.</p> |
